Results
| PMID | 21316665 |
| Gene Name | MBD2 |
| Condition | Endometriosis |
| Association |
Associated |
| Sex | Female |
| Associated genes | DNMT1, DNMT2, and DNMT3B and MBD1, MBD2, and MeCP2 |
| Other associated phenotypes |
Endometriosis |
|
Fertil Steril. 2011 Mar 15;95(4):1421-7. doi: 10.1016/j.fertnstert.2011.01.031. van Kaam, Kim J A F| Delvoux, Bert| Romano, Andrea| D'Hooghe, Thomas| Dunselman, Gerard A J| Groothuis, Patrick G GROW, School for Oncology and Developmental Biology, University of Maastricht, Maastricht, the Netherlands. kimvankaam@yahoo.com OBJECTIVE: To determine [1] expression levels of both DNA methyltransferases (DNMTs) and methyl-CpG-binding domain proteins (MBDs) in human endometrium throughout the menstrual cycle and in eutopic and ectopic endometrium of patients with endometriosis and [2] hormone responsiveness of DNMT and MBD expression in explant cultures of proliferative phase endometrium. DESIGN: In vitro study. SETTING: Academic medical center. PATIENT(S): Premenopausal women with and without endometriosis. INTERVENTION(S): Explant cultures of proliferative phase endometrium were treated with vehicle, 17beta-E(2), or a combination of E(2) and P (E(2) + P) for 24 hours. MAIN OUTCOME MEASURE(S): Expression levels of DNMT1, DNMT2, and DNMT3B and MBD1, MBD2, and MeCP2 with use of real-time quantitative polymerase chain reaction. RESULT(S): Expression levels of DNMT1 and MBD2 were significantly higher in secretory-phase endometrium compared with proliferative endometrium and menstrual endometrium. In explant cultures, treatment with E(2) + P resulted in significant up-regulation of DNMT1 and MBD2. Expression levels of several DNMTs and MBDs were significantly lower in endometriotic lesions compared with eutopic endometrium of women with endometriosis and disease-free controls. CONCLUSION(S): These findings suggest a role for DNMTs and MBDs in the growth and differentiation of the human endometrium and support the notion that endometriosis may be an epigenetic disease. Mesh Terms: DNA (Cytosine-5-)-Methyltransferase 1| DNA (Cytosine-5-)-Methyltransferases/*biosynthesis/genetics| DNA-Binding Proteins/*biosynthesis/genetics| Endometriosis/enzymology/genetics/*metabolism| Endometrium/enzymology/*metabolism/pathology| Female| Hum |
|